1/1/2024 0 Comments Massarray agena![]() ``Agena proactively sourced and secured materials to provide uninterrupted product availability to our customers. ``The unique characteristics of the Agena detection technology provide significant supply chain advantages,'' said Jason Halsey, Agena's senior vice president of technology and operations. Fragment analysis: The resulting fragments are subjected to MALDI-TOF analysis on a Agena MassARRAY Analyzer, where the mass difference of a fragment between an unmethylated and methylated CpG (16 Daltons, A to G) is resolved and quantified. The company says its panel targets five regions of the viral genome, and that with its authorization, the company hopes to help laboratories increase testing without the concerns of instrument or reagent availability. Custom panels for profiling CpG methylation can be designed using Agena’s EpiDesigner software. ![]() Here, we provide a guide to using the MassARRAY System, from assay design, through mass spectrometry, to generation of genotype. The high sensitivity of the MassARRAY System enables detection of differences as low as 5 between methylated and non-methylated DNA. The MassARRAY System is the perfect tool for that.'' The Agena Bioscience MassARRAY System is one such platform. ``To address the crisis presented by COVID-19, labs need to cost- effectively process large numbers of samples. ``Agena is proud to offer a truly scalable platform to support the increasing demand for high-throughput SARS-CoV-2 testing,'' said Agenda CEO Peter Dansky. Emergency Use Authorization has been granted to a large number of companies since the COVID-19 pandemic began for rapid approval of diagnostic tests, personal protective equipment, ventilators and other devices. The FDA's Emergency Use Authorization allows for the use of unapproved medical products during an emergency to diagnose, treat or prevent serious or life-threatening diseases or conditions. The company was granted approval Monday on an emergency basis for its MassARRAY SARS-CoV-2 panel for use in clinical laboratories. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.SAN DIEGO (CNS) - San Diego-based Agena Bioscience announced today that its diagnostic system for detection of SARS-CoV-2, the virus that causes COVID-19, received Emergency Use Authorization from the U.S. The 16-plex MRSA-iPLEX is a suitable alternative to SYBR Green-based real-time PCR typing of major sequence types and clonal complexes of MRSA. Agena Bioscience - Rapid & Accurate Genetic Testing Solutions One Platform, Endless Possibilities The MassARRAY System provides simplified genetic testing across a broad range of applications such as PGx, tumor profiling, liquid biopsy, inherited diseases, and more. MRSA-iPLEX provided optimal throughput for MRSA genotyping, and was, on a reagent basis, more cost-effective than the real-time PCR methods. The MassARRAY SARS-CoV-2 Panel is now available, with: ACCURATE DETECTION: Five SARS-CoV-2 assays multiplexed into a single reaction, targeting the nucleocapsid ( N) gene and ORF1ab region. ![]() Sequence variation in the MRSA-iPLEX primer targets accounted for the majority of MRSA-iPLEX erroneous results, highlighting the importance of primer target selection. CD Genomics offers a rapid and accurate custom SNP validation on the MassARRAY MALDITOF instrument provided by Agena Bioscience. With real-time PCR as the reference standard, MRSA-iPLEX correctly assigned 2298 of the 2352 (97.7%) markers. ![]() A total of 2352 markers belonging to 44 SNP-binary profiles were analysed by both real-time PCR and MRSA-iPLEX. The method was evaluated with 147 MRSA isolates, and the results were compared with those of an established SYBR Green-based real-time PCR system utilizing the same SNP-binary markers. A 16-plex MassARRAY iPLEX GOLD assay (MRSA-iPLEX) was developed that targets a set of informative SNPs and binary genes for MRSA characterization. In this study, we investigated the use of iPLEX MassARRAY technology for methicillin-resistant Staphylococcus aureus (MRSA) genotyping. Below are the different processing steps your samples are subjected to after we receive them here at the core facility. The Sequenom MassARRAY iPLEX single-nucleotide polymorphism (SNP) typing platform uses matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) coupled with single-base extension PCR for high-throughput multiplex SNP detection. How does it work For a detailed description of the chemistry used please consult Agena ( ). Sequenom has sold its Bioscience business for up-to-35.8 million to Agena Bioscience, which said it plans to expand clinical use of the MassARRAY® System for mass spectrometry-based detection. ![]()
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